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Proteomic analysis was undertaken to identify proteins from serum and A549 cells that bind to nano-Silicon Dioxide particles. Nano-SiO2 particles were incubated with serum-containing medium alone or in the presence of A549 cells under the same conditions as those used above to treat the cells. SDS-PAGE analysis of washed nano-Silicon Dioxide particles indicated selective binding of several proteins as evident from distinct protein bands in a stained gel, albeit over a background of numerous other proteins. Proteomic analysis indicated the presence of 18 distinct bovine proteins, but no human proteins. Similar results were found whether incubations were performed with bovine serum-containing medium alone or in the presence of human A549 cells. 

About to nano-Silicon Dioxide: the data suggest that only proteins from the serum, and not from the cells, bound to the nanoparticles in this study. Of the protein bands observed, the one at 16 kDa (row 9) was mainly a mixture of hemoglobin subunits alpha and gamma (i.e. fetal hemoglobin), the one at 25 kDa (row 7) was mainly apolipoprotein A-I, and the one at 65 kDa (row 4) appeared to be a mixture of alpha-1-antiproteinase and alpha-2-HS-glycoprotein as well as albumin and apolipoprotein A-I. Peptides corresponding to albumin and apolipoprotein A-I were found spread across several regions of the gel. Albumin peptides were found in every row examined. This may be the result of non-specific contamination by this highly abundant serum protein. Complement C3 was found in several rows. This protein is known to undergo proteolysis to produce a variety of mature proteins. Besides these proteins, there was also clear evidence of binding of several other serum glycoproteins including the cell adhesion proteins, fibronectin and victronectin.

Here we discuess nano-Silicon Dioxide (nm-SiO2) exposure on HaCaT cell membrane protein levels, screening nano-silica critical membrane proteins and membrane protein markers, for revealing the molecular mechanisms of toxicology of nano-silica provided experimental data and scientific value of clues for the prevention and treatment of the toxic effects of nano-silica provide new ideas. METHODS: Cultured HaCaT cells using adherent monolayer culture method, a membrane protein extraction kit HaCaT cell membrane proteins and nanoliter liquid chromatography were also used. Subsequently, with different concentrations of 15-nm nano silicon dioxide (nm-SiO2) on HaCaT cells were exposed to treatment, observe cell viability and cell morphology to determine exposure concentrations proteomics experiments. 


 

 

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