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The effect of nano-silicon dioxide particles was assessed further using different amounts of serum in the medium (serially diluted 2-fold from the original concentration of 10%) and from this a concentration of 1.25% serum was selected for more detailed assessment. In medium containing 1.25% serum and up to 10 μg/ml nano-SiO2 the cells maintained their morphology for 24 h. Cells treated with 100 μg/ml nano-SiO2 remained unaffected for 4 h although some morphological changes were apparent by 6 h and by 24 h marked changes were evident.

The condition of the cells was also examined by flow cytometry using a combination of PI and DiOC6 fluorescence measurements. A region of interest corresponding to the fluorescence characteristics of normal viable cells was defined and the number of cells in this region was estimated following treatment over time. In the absence of nano-silicon dioxide there was only a gradual and modest decrease in the number of cells in the region of interest, whether the cells were cultured in medium without serum or containing 1.25% serum.

Based on the surface of ultrafine silicon dioxide composite graft polymerization modification process, the situation before and after the modification of ultrafine silica has been studied, which to prove that the process can be achieved by ultrafine silica surface graft polymerization modified and effectively disperse ultrafine silica aggregates, the reason is styrene and pre-grafting on the surface of the silica ultrafine silane coupling agent of the double bond radical polymerization.


 

 

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