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Human lung-derived A549 cells were squamous in appearance as reported previously and when cultured in vitro grew as a monolayer of cells that adhered to the culture flask. The effect of nano-silicon dioxide particles on these cells was examined in culture for up to 24 h. The condition of the cells was assessed from their morphology (light microscopy), by flow cytometry analysis and their ability to metabolise MTT.

Initially, the cells were assessed using culture medium containing 10% serum. In the absence of nano-SiO2 particles the cells maintained a normal phenotype throughout the incubation period and were unaffected when exposed to up to1000 μg/ml nano-silicon dioxide particles. Thus, in the presence of 10% serum A549 cells appeared to be resistant to any cytotoxic effect of nano-SiO2 particles. This was evident in some cells after just 1 h in culture, and the fraction of cells affected increased with time, until no cells with the original morphology remained. Detached cells were recovered at each time point and placed in medium containing 10% serum to neutralise the cytotoxic effect of nano-SiO2. 

However, these cells failed to adhere or proliferate indicating they were no longer viable.By tetraethylorthosilicate hydrolysis and condensation, and adding an appropriate amount of a surfactant cetyl trimethyl ammonium bromide (CTAB), prepared nanometer silica (average particle size of 60 ~ 100 nm). Discussion formation mechanism of silicon dioxide and mechanism of action of CTAB, and the use of FTIR, XRD and TEM nano silica. 



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