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Southwest University of Science and solid waste treatment and recycling, Ministry of Education Key Laboratory researchers published papers, aimed to investigate the nano-Silicon Dioxide on the membrane oxidation boundary effects and mechanism of Escherichia coli. Research indicates that nano-silica oxide film on the E.coli sector mainly reflected in the free radical damage to the cell wall membrane damage and antioxidant systems. This article was published in 2015 on "Environment and Health."

E. coli were added to a suspension containing a final concentration of 0 (control) ~ 4 000μg / ml in PBS buffer nano-Silicon Dioxide incubated 24 h. By measuring nano-silica solution of hydroxyl radicals, cell survival, and cell wall surface binding group, intracellular reactive oxygen species, superoxide dismutase (SOD) and lactate dehydrogenase (LDH) Activity changes, the analysis of nano-silica limiting membrane of E. coli oxidation mechanism.

The results showed that compared with the control group, 1 000,2 000,4 000μg / ml nano-silica-exposed group were lower survival rate of E. coli, E. coli intracellular ROS content are high; different concentrations of nano-silica exposure group releasing hydroxyl radicals in PBS buffer levels were increased, E. higher LDH release of volume; 4 000μg / ml nano-silica-exposed group of E. coli intracellular SOD low vitality, the difference was statistically significant (P <0.05). And with the nano-Silicon Dioxide concentration increased, the system and the content of hydroxyl radicals and ROS release LDH amount of E. coli showed an upward trend, the survival rate and SOD in E. coli showed a downward trend. Cell wall surface phosphodiester group peak (1 078 cm-1 and 1 239 cm-1) disappeared protein molecule methyl symmetry (1 400 cm-1) bending vibration peak red shift.



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